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PCR Enzyme
  • AP211-11.JPG
AP211-11.JPG

EasyPfu DNA Polymerase  (含 2.5 mM dNTPs)


产品介绍

EasyPfu DNA Polymerase 是基因改造的Pfu DNA Polymerase,分子量为94 kDaEasyPfu DNA Polymerase 克服了普通Pfu酶扩增能力差、产量低的缺陷,增加了片段扩增长度,具有热启动功能。
• 保真性是EasyTaq
® DNA Polymerase 18倍。
• 具有5'→3' DNA 聚合酶活性和3'-5'外切酶活性。
• 扩增产物为平端,可直接克隆于pEASY®-Blunt 载体中
• 当以 cDNA 为模板时,MgSO4 工作浓度为3 mM
• 基因组DNA 片段的扩增(≤6 kb)。
• Plasmid DNA片段扩增(≤10 kb)。

References

Zhao J , Liu Q , Hu P , et al. An efficient Potato virus X -based microRNA silencing in Nicotiana benthamiana[J]. Scientific Reports, 2016, 6(1):20573.

Ding K , Pei T , Bai Z , et al. SmMYB36, a Novel R2R3-MYB Transcription Factor, Enhances Tanshinone Accumulation and Decreases Phenolic Acid Content in 
Salvia miltiorrhiza Hairy Roots[J]. Scientific Reports, 2017, 7(1):5104

Guo X , Fan C , Chen Y , et al. Identification and characterization of an efficient acyl-CoA: diacylglycerol acyltransferase 1 ( DGAT1 ) gene from the microalga 
Chlorella ellipsoidea[J]. Bmc Plant Biology, 2017, 17(1):48.

Meng D, Shen L, Yang R, et al. Identification and active site analysis of the 1-aminocyclopropane-1-carboxylic acid oxidase catalysing the synthesis of 
ethylene in Agaricus bisporus[J].Biochim Biophys Acta. 2014, 1840(1):120-8.

Zheng T , Huang Q , Zhang C , et al. Development of a Simvastatin Selection Marker for a Hyperthermophilic Acidophile, Sulfolobus islandicus[J]. Applied 
and Environmental Microbiology, 2012, 78(2):568-574.


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