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蛋白检测
  • DQ101-01.JPG
DQ101-01.JPG

Easy Protein Quantitative Kit (Bradford)


产品介绍

Bradford 蛋白定量方法,原理是在酸性条件下,考马斯亮蓝G-250 和蛋白质结合产生特殊的蓝色,最大吸收波长在595 nm 处,颜色反应可5-10 分钟内完成,20 分钟内最稳定,测定效果最好。因此可通过比色分析在几分钟内快速测定蛋白质含量。与其它蛋白定量方法(如Lowry 法)相比,该方法测定蛋白浓度不受样品中化学物质的影响。

产品组成

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References

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Zhang K , Zhao W J , Leng X Y , et al. The importance of the last strand at the C-terminus in βB2-crystallin stability and assembly[J]. Biochimica et Biophysica Acta (BBA) - Molecular Basis of Disease, 2014, 1842(1):44-55.

Yu-Rong L , Wen-Xiu M , Yi-Zhou C , et al. A Highly-Conserved Single-Stranded DNA-Binding Protein in Xanthomonas Functions as a Harpin-Like Protein to Trigger Plant Immunity[J]. PLoS ONE, 2013, 8(2):e56240.

Fang-Cheng B , Quan-Fang Z , Zhe L , et al. A Conserved Cysteine Motif Is Critical for Rice Ceramide Kinase Activity and Function[J]. PLoS ONE, 2011, 6(3):e18079.

Che Y Z , Li Y R , Zou H S , et al. A novel antimicrobial protein for plant protection consisting of a Xanthomonas oryzae harpin and active domains of cecropin A and melittin.[M]. Microbial Biotechnology. 2011, 4(6):777-93.