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蛋白检测
  • DQ111-01.JPG
DQ111-01.JPG

Easy II Protein Quantitative Kit (BCA)


产品介绍

BCA蛋白定量方法是目前最常用的两种蛋白定量方法之一,其原理是在碱性条件下,蛋白质中的肽键能将Cu2+还原成Cu1+(双缩脲反应),且Cu2+的还原量与总蛋白量成正比,生成的Cu1+能够与BCA形成稳定的紫色化合物。与Bradford方法相比,BCA蛋白定量方法更加客观,普通的肽键即可发生颜色反应,亦可兼容高浓度的SDS(5%),Triton X-100(5%),NP 40(5%),CHAPS(5%),Tween 20,60,80(5%),其弊端是易受某些还原剂(如二硫苏糖醇和β-硫基乙醇等)、螯合剂(EDTA,EGTA)和高浓度缓冲液的影响,但这些干扰作用可以通过对蛋白质样品的稀释进行消除。  

产品组成

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References

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